Starch Hydrolysis Test

Starch Hydrolysis Test

The biochemical test starch hydrolysis test is used to determine if an organism is able to hydrolyze starch by producing an exoenzyme called amylase. The absence or presence of starch in the medium is detected by using iodine solution as an indicator.

Nitrate Reduction Test

Nitrate Reduction Test

The nitrate reduction test is a biochemical test with the application to determine the ability of microorganisms to reduce nitrate to nitrite or free nitrogen gas. In other words, this test is used to test the presence of the enzyme nitrate reductase.

Oxidase test

Oxidase test

The oxidase test is a biochemical test used to determine the presence of oxidase enzyme in microorganisms as this enzyme is capable of catalyzing the transport of electrons between a redox dye tetramethyl-p-phenylene-diamine and electron donors.

Catalase test

Catalase test

The biochemical test catalase test is used to determine if the test microorganism can produce the catalase enzyme which is able to decompose hydrogen peroxide to release oxygen and water.

Citrate Test

Citrate Test

The citrate utilization test is a biochemical test used to determine if the test organism is able to metabolize citrate as the sole of carbon and energy with resulting alkalinity.

Voges-Proskauer Test

Voges-Proskauer Test

Voges-Proskauer (VP) test is a biochemical test used in the differentiation/identifying bacteria by detecting the presence or absence of acetoin (acetylmethyl carbinol).

Methyl Red Test, MR

Methyl Red Test, MR

The Methyl red (MR) test is used to test the ability of an organism to produce and maintain stable adequate acid end products when it utilizes glucose through fermentation.

Indole test

Indole test

The indole test is a biochemical test used to determine if the test bacteria are able to convert the α-amino acid tryptophan into indole, an aromatic heterocyclic organic compound.