Laboratory diagnosis of fungal infection begins with sample collection, transportation and processing of samples.

Wet Mount Method for fungi is done for identification of fungi for numerous reasons.

Slide Culture for fungi was first developed in 1950 by Riddel.

Scotch tape preparation or tape touch mount is done to microscopically study molds.

The cell wall is a structural layer surrounding some cell types.

For fungal culture, a high carbohydrate and nitrogen source is essential with a pH range from 5 to 6 and a temperature range from 15 to 37ºC.

This method is a gold standard in mycology.

Since Wright’s stain easily distinguishes between blood cells, this procedure became popular for differential WBC counts and the study of the morphology of blood cells.

Calcofluor White (CFW) is a non-specific fluorescent blue dye used in biology as well as textiles.

Laboratory diagnosis of fungal infection is highly dependent on the quality of specimens.