Giardia intestinalis - Classification, History, Habitat, Morphology, Culture

Introduction of Giardia intestinalis

Giardia intestinalis, also known as G. lamblia or G. duodenalis inhabits the intestinal tract of humans. They are the only intestinal parasitic flagellate known to cause both endemic and epidemic diarrhea in humans.

Classification of Giardia intestinalis

Giardia intestinalis is classified:

Domain: Eukaryota

Phylum: Metamonada

Order: Diplomonadida

Family: Hexamitidae

Subfamily: Giardiinae

Genus: Giardia

Species: intestinalis

History of Giardia intestinalis

Histologically, in 1681, Antony von Leeuwenhoek first observed the flagellate when examining his own stool. Lambl coined the name Giardia lamblia in 1859 after observing it in human stool. Only in the 1970s, the flagellate was recognized as a parasite as protozoa were considered a commensal organism before this.

Habitat of Giardia intestinalis

Giardia intestinalis inhabits the small intestine of humans and the trophozoites, cysts are found in the duodenum, jejunum, and upper ileum of the intestine.

Morphology of Giardia intestinalis

The Giardia intestinalis parasite occurs in two stages- trophozoites and cysts

Trophozoite

• this morphological form of Giardia intestinalis is 9μm – 21 μm in length and 5μm- 15μm in breadth

• trophozoites are pear-shaped, bilateral bodes with a broad rounded anterior end and a tapering posterior end

• since their dorsal surface is convex and ventral surface is concave, they appear sickle-shaped when viewed from a lateral view

• it bears two median bodies, two axonemes, four pairs of flagella (two pairs lateral, one pair ventral, and one pair caudal), two nuclei

• each of the two nuclei contains a larger central karyosome

• the axostyles run diagonally across the cytoplasm which is uniform and finely granular

• a bilobed adhesive disc is present on one-third to one-half of the ventral surface

  * These discs are rigid structures that help in attachment to the host cells

• giardin is a tubulin intermediate filament that is a part of the cytoplasmic extension

• median bodies are a pair of large, curved, and transverse bodies present behind the adhesive discs

  Although the function of the median bodies is unclear, it is believed to help in energy metabolism

• motility takes place with the help of flagella, showing the typical “falling leaf motility”

Cyst

• cyst is the infective form of G. lamblia

• oval or ellipsoidal in shape, measuring 8μm-12μm

• a thick, finely granular cytoplasm cyst wall surrounds the cyst and is separated from the cyst wall by a thin, clear space

• it contains four nuclei, axostyle, and median bodies

• if stained with iodine, the cysts take brown color

Culture of Giardia intestinalis

Giardia intestinalis can be cultured in media as well as laboratory animals.

In media

Giardia intestinalis can be grown axenically in Diamond’s medium, containing Entamoeba histolytica. It is mostly used for research purposes and rarely for routine diagnosis

In animals

Giardia intestinalis is inoculated into laboratory mice to study the host immune response and pathogenesis of malabsorption in giardiasis.