Triple Sugar Iron Agar test, TSIA

Triple Sugar Iron Agar test, TSIA

The biochemical test triple sugar iron agar (TSIA) test is used to determine the ability of the organism to produce gas from sugar fermentation, production of hydrogen sulfide gas, and ability to ferment lactose and sucrose.

Oxidation Fermentation Test

Oxidation Fermentation Test

The oxidation fermentation test is a biochemical test used to determine/differentiate the ability of microorganisms to metabolize carbohydrates and related compounds. The OF medium can be used to differentiate such a group of organisms on the basis of carbohydrate utilization.

Fat, Lipid Hydrolysis Test

Fat, Lipid Hydrolysis Test

The fat/lipid hydrolysis test is a biochemical test used in the laboratory to determine if the test organism is able to produce lipase (esterase) that cleaves its ester bonds to produce glycerol and fatty acids. Such organisms are called lipolytic organisms.

Starch Hydrolysis Test

Starch Hydrolysis Test

The biochemical test starch hydrolysis test is used to determine if an organism is able to hydrolyze starch by producing an exoenzyme called amylase. The absence or presence of starch in the medium is detected by using iodine solution as an indicator.

Nitrate Reduction Test

Nitrate Reduction Test

The nitrate reduction test is a biochemical test with the application to determine the ability of microorganisms to reduce nitrate to nitrite or free nitrogen gas. In other words, this test is used to test the presence of the enzyme nitrate reductase.

Oxidase test

Oxidase test

The oxidase test is a biochemical test used to determine the presence of oxidase enzyme in microorganisms as this enzyme is capable of catalyzing the transport of electrons between a redox dye tetramethyl-p-phenylene-diamine and electron donors.

Catalase test

Catalase test

The biochemical test catalase test is used to determine if the test microorganism can produce the catalase enzyme which is able to decompose hydrogen peroxide to release oxygen and water.

Citrate Test

Citrate Test

The citrate utilization test is a biochemical test used to determine if the test organism is able to metabolize citrate as the sole of carbon and energy with resulting alkalinity.

Voges-Proskauer Test

Voges-Proskauer Test

Voges-Proskauer (VP) test is a biochemical test used in the differentiation/identifying bacteria by detecting the presence or absence of acetoin (acetylmethyl carbinol).

Methyl Red Test, MR

Methyl Red Test, MR

The Methyl red (MR) test is used to test the ability of an organism to produce and maintain stable adequate acid end products when it utilizes glucose through fermentation.