Indole test

Indole test

The indole test is a biochemical test used to determine if the test bacteria are able to convert the α-amino acid tryptophan into indole, an aromatic heterocyclic organic compound.

Mantoux test

Mantoux test

Mantoux test is used for screening as well as diagnosis of tuberculosis caused by Mycobacterium tuberculosis. After infection by M. tuberculosis, the individual develops a delayed hypersensitivity to tuberculin.

Elek's plate test

Elek's plate test

Elek's test (also known as Elek plate test or Elek gel precipitation test) is an invitro test to test the virulence of Corynebacterium diphtheriae, the causative agent of the infection diphtheria.

X factor, V factor test

X factor, V factor test

X factor (hemin) and V factor (NAD, NADP) tests are done for the identification and/or differentiation of Haemophilus spp., especially the important human pathogen Haemophilus influenzae.

Satellitism test

Satellitism test

The Satellitism test is used to identify/differentiate Haemophilus spp. as they require X factor (hemin) and V factor (NAD) for growth. Some species require both factors while others grow well with only one.

DNase test, DNA hydrolysis

DNase test, DNA hydrolysis

DNase test / DNA hydrolysis is used to determine if the test microorganism (bacteria, fungi) is able to hydrolyze DNA and utilize the nucleotides as a source of carbon, phosphate, nitrogen, and energy for growth.

Butyrate Disk test

Butyrate Disk test

The butyrate disk test (Tri-butyryl esterase test) is a rapid test used to detect the production of the enzyme butyrate esterase. This test is important identification Moraxella catarrhalis and differentiate it from Neisseria gonorrhea.

CAMP test

CAMP test

The CAMP (Christie–Atkins–Munch-Peterson) test is based on the microorganism's formation of the CAMP factor that enlarges the area of β-hemolysis due to the production of β-hemolysin manufactured by Staphylococcus aureus.

Pyrrolidonyl Arylamidase test, PYR

Pyrrolidonyl Arylamidase test, PYR

The objective of Pyrrolidonyl Arylamidase/Aminopeptidase (PYR) test is to detect the presence of pyrrolidonyl arylamidase (also known as pyrrolidonyl aminopeptidase or L-pyrrolidonyl-β-naphthylamide) in microorganisms.

Hippurate hydrolysis test

Hippurate hydrolysis test

Hippurate hydrolase is an enzyme that reversibly catalyzes Hippurate into sodium benzoate and glycine in the presence of water. Hippurate hydrolysis test uses this principle and adds ninhydrin which deaminates glycine to give purple color.