Acanthamoeba

Acanthamoeba

Acanthamoeba is a genus of amoebae that can cause infections called acanthamebiasis in humans as well as animals.

Naegleria fowleri

Naegleria fowleri

It causes primary amoebic meningoencephalitis (PAM) which is an acute, fulminant, rapid, and fatal infection of the central nervous system.

Entamoeba histolytica

Entamoeba histolytica

The parasitic protozoa Entamoeba histolytica causes the disease amoebiasis. It predominantly infects humans as well as other primates and is worldwide in distribution.

Pour Plate Technique

Pour Plate Technique

The pour plate technique is based on the principle that when a single viable microbial cell that has been separated by dispersion from one another in a confined space of an agar medium will give rise to a macroscopically visible separate colony.

Miles and Mishra Enumeration

Miles and Mishra Enumeration

The Miles and Mishra enumeration or Surface Drop method is a Surface Viable Count (SVC) method that involves placing drops of (0.02ml) of test microorganisms on the surface of a solid agar plate medium followed by counting the number of colonies that grow on the inoculated agar after incubation.

Spread Plate Enumeration

Spread Plate Enumeration

Spread plate enumeration is a type of plate count method or standard count method of enumeration of microorganisms that only counts viable cells. In addition to enumeration, it also isolates the organisms from a mixed culture.

Enumeration by Counting Chamber

Enumeration by Counting Chamber

Counting Chamber counts the number of microbes present in a small known volume of sample and is applied to determine the number of bacteria in the larger original sample. Hemocytometers such as Petroff Hauser Chamber or Superior Marienfeld Hemocytometer can be used.

Breed Count Method

Breed Count Method

The breed count method detects the number of both viable and non-viable bacteria, algae, or yeast cells present in samples such as broth culture, beer, fruit juices, milk, and water. It is a direct cell count method and is done by direct microscopic examination.

Microbial Cell Enumeration

Microbial Cell Enumeration

The number of microorganisms at any given time can be determined by following the course of the growth cycle. As exponential growth is a balanced phase, the growth rate is determined by the properties of cell mass and cell number.

Motility Culture Method

Motility Culture Method

Like mother motility test methods, the culture method is used in the identification process since most gram-negative bacteria are motile in mostly pathogenic microbes.